Instrument Plus | Instrument Introduction: Nucleic Acid Extraction and Purification _ Operation

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Original Title: Instrument Plus | Instrument Introduction: Nucleic Acid Extraction and Purification Introduction of Nucleic Acid Extraction and Purification Nucleic acid is one of the most basic substances of life, which can be divided into DNA and RNA, and widely exists in all living organisms such as animals, 5. Safe, non-toxic and harmless The reagent does not contain toxic chemical reagents such as phenol and chloroform, which fully conforms to the modern environmental protection concept. Precautions for Nucleic Acid Extraction by Magnetic Bead Method The use of biological magnetic beads to extract nucleic acid is still a relatively new method of nucleic acid extraction in China. Compared with the traditional isoamyl alcohol extraction method and centrifugal column kit method,rotovap distillation, this method is still inadvertently understood by many people. In the process of using magnetic beads to purify nucleic acid, there are also some misunderstandings. 1. Mistake one : The more magnetic beads you use, the better the extraction Many teachers like to increase the amount of magnetic beads when the extraction effect is not good, thinking that more magnetic beads can absorb more nucleic acid, which has to be said to be undesirable. The main characteristic of magnetic beads is that they can be dispersed in liquid, and can also be separated from liquid in solid state under the action of external magnetic field. In any reagent system, the ratio of magnetic beads to liquid should have a certain threshold. If the ratio exceeds a certain threshold, too many magnetic beads will lose their dispersion characteristics because they can not be uniformly dispersed in liquid. The efficiency of the contact between the nucleic acid magnetic beads and the liquid cannot be sufficiently increased during the washing process. Excessive magnetic beads will also adsorb more impurities, which has a great impact on the effect of impurity removal. Even sometimes, too many magnetic beads will adsorb protease, lysozyme and other functional components that play a major role in the liquid system, resulting in low efficiency of the whole kit. In many cases, when the extraction effect is not good, reducing the use of magnetic beads is the best way to improve the extraction effect. Usually, the amount of reference magnetic beads given by the magnetic bead method kit is slightly excessive, so it is not necessary to increase the amount of magnetic beads to improve the adsorption efficiency. However, if it is determined that the extraction effect is not good due to insufficient amount of magnetic beads, it is possible to improve the extraction effect by increasing the dosage of magnetic beads within a certain range. Taking GNT-02 series magnetic beads as an example, when extracting constant samples (plant tissues, whole blood, etc.),wiped film evaporator, the dosage is usually 10 UL/time; when extracting trace samples (such as serum free DNA, oral swabs, etc.), the dosage of magnetic beads is 15 ~ 20 UL/time. If you need to exceed this usage, you need to communicate with the technical engineer. 2. Mistake two The more reagents are used, the better the extraction effect is. The cracking effect is not good? Add more lysis solution. Therefore, different magnetic beads are suitable for different experiments and systems. Just like the same reagent for nucleic acid extraction, the formula is not exactly the same, cbd centrifugal extractor ,thin film distillation, and the properties of magnetic beads used for nucleic acid extraction are not exactly the same. Some magnetic beads show higher adsorption efficiency in the extraction of normal nucleic acid, and some magnetic beads are more suitable for the extraction of trace nucleic acid. Some magnetic beads are suitable for acidic series of reagent systems, and some magnetic beads are suitable for alkaline series of reagent systems. Instrument and Equipment Test Special statement: The publication of this article is only for the purpose of disseminating information, and does not represent the views of this public account; if other media, websites or individuals reprint and use from this public account, please apply to the original author, and bear the copyright and other legal responsibilities. Instrument plus | dry goods finishing: chromatographic column use,winterization filtration, maintenance and repair, all for you to organize together ~ do not collect it? Instrument Plus | News and Information: Southern Hemisphere Aerosol Content Breaks Record, Air Pollution Needs Urgent Attention! Instrument Plus | Dry Goods Finishing: Liquid Chromatography Basic Concepts and Terminology! Return to Sohu to see more Responsible Editor:. toptiontech.com

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